SARS-CoV-2 Nucleocapsid antibody

Catalog No. ABIN6953169

The Mouse Monoclonal anti-SARS-CoV-2 Nucleocapsid antibody has been validated for ELISA, WB and LF. It is suitable to detect SARS-CoV-2 Nucleocapsid in samples from SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha, SARS CoV-2 Beta, SARS CoV-2 Delta, SARS CoV-2 Gamma and SARS CoV-2 Omicron.

Quick Overview for SARS-CoV-2 Nucleocapsid antibody (ABIN6953169)

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Reactivity: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha, SARS CoV-2 Beta, SARS CoV-2 Delta, SARS CoV-2 Gamma, SARS CoV-2 Omicron

Host: Mouse

Clonality: Monoclonal

Conjugate: This SARS-CoV-2 Nucleocapsid antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Lateral Flow (LF)

Product Details anti-SARS-CoV-2 Nucleocapsid Antibody

Purpose: Monoclonal Antibody to SARS-CoV-2 Nucleocapsid Protein

Specificity: This antibody and its recommended antibody pair have been proven to detect all known SARS-CoV-2 variants such as Omicron variant (B1.1.529), Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), and Delta (B.1.617.2). This antibody is used in several regulated immunoassays worldwide.

Cross-Reactivity (Details): This antibody does not cross react with:

• MERS-coronavirus
• Human coronavirus (NL63, 229E, OC43)
• Human Adenovirus (type 1, 3, 5, 7, 8, 11, 18, 23)
• Human Parainfluenza virus (type 1, 2, 3, 4)
• Human Rhinovirus (type 1, 14, 42)
• Human Metapneumovirus
• Respiratory syncytial virus-A
• Respiratory syncytial virus-B

This antibody does cross react with SARS-CoV.

Characteristics: The performance of a rapid antigen test is limited by the sensitivity of the antibodies used. The new pair of monoclonal antibodies is highly sensitive for the detection of SARS-CoV-2 Nucleoprotein and they do not cross-react with seasonal coronavirus strains. They are ideal for developing reliable and sensitive rapid lateral flow antigen assays for the detection of active COVID-19 infections.

Purification: Protein A Chromatography

Purity: > 95 % (SDS-PAGE)

Immunogen: Recombinant SARS-CoV-2 Nucleocapsid Protein

Isotype: IgG1

Application Details

Application Notes: Each laboratory should determine an optimum working titer for use in its particular application.

Recommended pair for Immunoassay:

Capture - Detection

• ABIN6953170 - ABIN6953169
• ABIN6953170 - ABIN6953295

Comment:

An FDA-approved COVID-19 lateral flow assay using the SARS-CoV-2 nucleocapsid antibody pair (ABIN6953170 + ABIN6953169 ) shows that the pair can detect the Omicron variant (live virus of B.1.1.529) up to 1.31 x 10^2 PFU/mL, and other variants up to 1.88 x 10^2 PFU/mL. The two antibodies recognize the conserved nucleoprotein domain of SARS-CoV-2 and are already used in many commercially available COVID-19 antigen rapid tests around the world.

These two antibodies work as a High-Sensitivity SARS-CoV-2 Nucleoprotein Antibody Pair for Rapid COVID-19 Antigen Assays: ABIN6953170, ABIN6953169

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 4.8 mg/mL

Buffer: Phosphate Buffered Saline, pH 7.4, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C

Target Details for SARS-CoV-2 Nucleocapsid

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Alternative Name: SARS-CoV-2 Nucleocapsid Protein

Target Type: Viral Protein