Recombinant mScarlet antibody (Alexa Fluor 647)

Catalog No. ABIN6953250

Product Details

Target: mScarlet

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Dictyosoma

Host: Alpaca

Expression System: E.coli

Clonality: Monoclonal

Conjugate: Alexa Fluor 647

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: Camelid sdAb anti-mScarlet-i conjugated with Alexa647, Clone 2B12

Specificity: Recognizes mScarlet-I and most fluorescent proteins from Dictiosoma sp. and most common RFP derivatives

Cross-Reactivity (Details): Does not cross-react with GFP, CFP, YFP and mTagBFP or their most common derivatives.

Characteristics: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Purification: Produced in: E.coli

Clone: 2B12

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Application Details

Application Notes: Recommended dilution 1:500

Comment:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 5 μM

Buffer: 2.5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light!

Storage: -20 °C,-80 °C

Storage Comment: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Expiry Date: 12 months

Target Details

Target: mScarlet