CD28 antibody

Catalog No. ABIN6961445

This Mouse Monoclonal antibody specifically detects CD28 in FACS, WB and Func. It exhibits reactivity toward Human.

Quick Overview for CD28 antibody (ABIN6961445)

Target: CD28

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func)

Clone: CD28-6

Product Details anti-CD28 Antibody

Cross-Reactivity: Human

Purification: This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.

Endotoxin Level: Low endotoxin: < 0.01 EU/ug

Isotype: IgG2a kappa

Application Details

Application Notes: This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.

Comment:

2 mg/mL

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, pH 7.2

Preservative: Azide free

Storage: 4 °C

Storage Comment: 2-8°C

Expiry Date: 12 months

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: The CD28.6 antibody reacts with human CD28, a 44 kDa type I surface glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), which are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. The CD28.6 antibody is reported to have neutralizing activity and is also useful for western blotting.

Gene ID: 940

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response