ICOSLG antibody (PE)

Catalog No. ABIN7278355

The Rat Monoclonal anti-ICOSLG antibody has been validated for FACS. It is suitable to detect ICOSLG in samples from Mouse.

Quick Overview for ICOSLG antibody (PE) (ABIN7278355)

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ICOSLG antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: HK5-3

Product Details anti-ICOSLG Antibody (PE)

Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.

Comment:

0.2 mg/mL

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C protected from light

Expiry Date: 12 months

Target Details for ICOSLG

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Alternative Name: CD275 (B7-H2)

Background: The HK5.3 antibody reacts with mouse CD275, an approximately 40 kDa protein that is a member of the Ig superfamily. CD275 is also known as B7-H2, ICOS ligand, and B7-RP1. CD275 is a ligand for ICOS (CD278) that is expressed on activated T cells. CD275 is expressed by antigen presenting cells including B cells, macrophages, and dendritic cells. The interaction of CD275 with its receptor ICOS plays an important role in T cell costimulation.

Gene ID: 50723

UniProt: Q9JHJ8

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation