CD32/CD16 antibody

Catalog No. ABIN6961728

The Rat Monoclonal anti-CD32/CD16 antibody is suitable to detect CD32/CD16 in samples from Mouse. It has been validated for FACS, IP and IF.

Quick Overview for CD32/CD16 antibody (ABIN6961728)

Target: CD32/CD16

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD32/CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 2-4G2

Product Details anti-CD32/CD16 Antibody

Purification: This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.

Isotype: IgG2b

Application Details

Application Notes: This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.

Comment:

0.5 mg/mL

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

Expiry Date: 12 months

Target Details for CD32/CD16

Target: CD32/CD16

Alternative Name: CD16 / CD32

Background: The 2.4G2 antibody is specific for a common epitope found in the extracellular regions of mouse Fc-receptors Fc-gamma II (CD32) and Fc-gamma III (CD16). As these are receptors for the Fc portion of mouse IgG, they may also bind laboratory antibody preparations and products used in a variety of cell analysis protocols such as flow cytometry, immunohistochemistry and functional cell assays. The 2.4G2 antibody is therefore widely used as a pre-treatment reagent to block binding of specific antibodies of interest, e.g. fluorescently conju- gated antibodies, to Fc receptors via their Fc domains and contributing to ""non-specific"" staining.

Gene ID: 14130, 14131