IL7R antibody

Catalog No. ABIN6961754

This Rat Monoclonal antibody specifically detects IL7R in FACS, IP and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for IL7R antibody (ABIN6961754)

Target: IL7R (Interleukin 7 Receptor (IL7R))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL7R antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: A7R34

Product Details anti-IL7R Antibody

Purification: This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.

Isotype: IgG2a kappa

Application Details

Application Notes: This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.

Comment:

0.5 mg/mL

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

Expiry Date: 12 months

Target Details for IL7R

Target: IL7R (Interleukin 7 Receptor (IL7R))

Alternative Name: CD127 (IL-7Ra)

Background: The A7R34 antibody is specific for mouse CD127, a 60-90 kDa cell surface protein also known as the Interleukin-7 Receptor alpha chain, or IL-7R alpha. CD127 is typically expressed at the cell surface as a heterodimer with the common gamma chain (CD132). This complex acts as the functional receptor for IL-7, a cytokine important in T and B cell development, and in mature T cell homeostasis. A second cytokine known as Thymic Stromal Lymphopoietin (TSLP) also binds to a receptor complex of CD127 and the TSLPR chain to trigger activation of dendritic cells, and is involved in B cell development, allergy and autoimmunity.The A7R34 antibody may be used as a phenotypic marker for CD127 on immature B cells, on subsets of thymocytes which are double negative (CD4-CD8- ) or single positive (CD4+ or CD8+), and at low levels on mature, peripheral T cells. CD127 is a key marker, when used in combination with CD4 and CD25, to distinguish Treg and effector/memory Treg populations known as T(REM).

Gene ID: 16172

UniProt: P16872

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size