CD16 antibody (violetFluor™ 450)

Catalog No. ABIN7278618

The Mouse Monoclonal anti-CD16 antibody has been validated for FACS. It is suitable to detect CD16 in samples from Human.

Quick Overview for CD16 antibody (violetFluor™ 450) (ABIN7278618)

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is conjugated to violetFluor™ 450

Application: Flow Cytometry (FACS)

Clone: 3G8

Product Details anti-CD16 Antibody (violetFluor™ 450)

Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 μL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells. violetFluor™ 450 dye is excited by the violet (405 nm) laser and has a peak emission of 450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluor™ 450 can be used as an alternative for Pacific Blue®, BD Horizon™ V450 or eFluor® 450.

Comment:

5 μL (0.5 μg)/test

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C protected from light

Expiry Date: 12 months

Target Details for CD16

Target: CD16

Alternative Name: CD16

Background: The 3G8 monoclonal antibody reacts with the 50-65kD transmembrane form of human CD16 (FCGR3A). CD16 is the low affinity IgG receptor III and is expressed on NK cells and macrophages. CD16 participates in signal transduction and mediates antibody-dependent cellular cytotoxicity (ADCC) by natural killer (NK) cells. The second form of CD16 (FCGR3B) is a glycosyl-phosphatidylinositol (GPI) linked molecule expressed exclusively on neutrophils.

Gene ID: 2214, 2215

UniProt: P08637, O75015

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process