Ly6g antibody (violetFluor™ 450)

Catalog No. ABIN7278566

This Rat Monoclonal antibody specifically detects Ly6g in FACS. It exhibits reactivity toward Mouse.

Quick Overview for Ly6g antibody (violetFluor™ 450) (ABIN7278566)

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Ly6g antibody is conjugated to violetFluor™ 450

Application: Flow Cytometry (FACS)

Clone: 1A8

Product Details anti-Ly6g Antibody (violetFluor™ 450)

Purification: This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indi- cated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. violetFluor™ 450 dye is excited by the violet (405 nm) laser and has a peak emission of 450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluor™ 450 can be used as an alternative for Pacific Blue®, BD Horizon™ V450 or eFluor® 450.

Comment:

0.2 mg/mL

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C protected from light

Expiry Date: 12 months

Target Details for Ly6g

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Alternative Name: Ly-6G

Background: The 1A8 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the 1A8 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: for identification of Ly-C, an alternative antibody, clone RB6-8C5, has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C.

Gene ID: 546644