CD3 antibody (violetFluor™ 500)

Catalog No. ABIN7278611

This anti-CD3 antibody is a Mouse Monoclonal antibody detecting CD3 in FACS. Suitable for Human.

Quick Overview for CD3 antibody (violetFluor™ 500) (ABIN7278611)

Target: CD3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to violetFluor™ 500

Application: Flow Cytometry (FACS)

Clone: UCHT1

Product Details anti-CD3 Antibody (violetFluor™ 500)

Cross-Reactivity: Chimpanzee

Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μLper test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells. violetFluor™ 500 dye is excited by the violet (405 nm) laser and has a peak emission of 500 nm. The recommended band pass filter for this dye is 525/20. violetFluor™ 500 can be used as an alternative for BD Horizon™ V500 or Pacific Orange®.

Comment:

5 uL (0.5 ug)/test

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C protected from light

Expiry Date: 12 months

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: The UCHT1 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex. Together with CD3 gamma, CD3 delta and CD3 zeta, CD3 epsilon assembles to form the CD3 complex that associates with the T cell receptor (TCR). Along with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte development and on all mature T cells.The UCHT1 antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of UCHT1 antibody to CD3e can induce cell activation (use format suitable for functional assays).

Gene ID: 915, 916, 917

UniProt: P20963, A8MVP6, P07766, P09693

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway