Interferon gamma antibody (Biotin)

Catalog No. ABIN6963762

The Mouse Monoclonal anti-Interferon gamma antibody has been validated for ELISA and ELISpot. It is suitable to detect Interferon gamma in samples from Dog. There are 2+ publications available.

Quick Overview for Interferon gamma antibody (Biotin) (ABIN6963762)

Target: Interferon gamma (IFNG)

Reactivity: Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is conjugated to Biotin

Application: ELISA, ELISpot

Clone: MT166

Product Details anti-Interferon gamma Antibody (Biotin)

Specificity: Native and recombinant IFN-γ

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IFN-γ from horse.

Characteristics: This monoclonal antibody enables specific detection of canine IFN-γ in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant equine IFN-gamma

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963762, ABIN7448233, ABIN6963768, ABIN7448239, ABIN6963770 and ABIN6963771 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb ABIN6963761, ABIN7448232, ABIN6963769 and ABIN7448240 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-Interferon gamma antibody (Biotin) (ABIN6963762)

Li, Wu, Zou, Zhai, Chen, Bu, Chen, Luo: "Expression and characterization of albumin fusion protein canine IFNγ-CSA in baculovirus-insect cell expression system." in: Protein expression and purification, Vol. 162, pp. 32-37, (2020) (PubMed).

Li, Chen, Zou, Wu, Wang, Chen, Luo: "Albumin fusion improves the pharmacokinetics and in vivo antitumor efficacy of canine interferon gamma." in: International journal of pharmaceutics, Vol. 558, pp. 404-412, (2019) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Gene ID: 403801

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy