Interferon gamma antibody

Catalog No. ABIN6963768

The Mouse Monoclonal anti-Interferon gamma antibody has been validated for ELISA and ELISpot. It is suitable to detect Interferon gamma in samples from Horse and Rhinoceros. There are 3+ publications available.

Quick Overview for Interferon gamma antibody (ABIN6963768)

Target: Interferon gamma (IFNG)

Reactivity: Horse, Rhinoceros

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: ELISA, ELISpot

Clone: MT166

Product Details anti-Interferon gamma Antibody

Specificity: Native and recombinant IFN-γ

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IFN-γ from dog rhinoceros.

Characteristics: This monoclonal antibody enables specific detection of equine IFN-γ in immunoassays such as ELISpot and ELISA. Serum/Plasma samples

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant equine IFN-gamma

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963762, ABIN7448233, ABIN6963768, ABIN7448239, ABIN6963770 and ABIN6963771 is recommended as capture mAb in ELISpot and ELISA in combination with detection mAb ABIN6963761, ABIN7448232, ABIN6963769 and ABIN7448240 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-Interferon gamma antibody (ABIN6963768)

Cywes-Bentley, Rocha, Bordin, Vinacur, Rehman, Zaidi, Meyer, Anthony, Lambert, Vlock, Giguère, Cohen, Pier: "Antibody to Poly-N-acetyl glucosamine provides protection against intracellular pathogens: Mechanism of action and validation in horse foals challenged with Rhodococcus equi." in: PLoS pathogens, Vol. 14, Issue 7, pp. e1007160, (2019) (PubMed).

Hellman, Hjertner, Morein, Fossum: "The adjuvant G3 promotes a Th1 polarizing innate immune response in equine PBMC." in: Veterinary research, Vol. 49, Issue 1, pp. 108, (2018) (PubMed).

Rocha, Cohen, Bordin, Brake, Giguère, Coleman, Alaniz, Lawhon, Mwangi, Pillai: "Oral Administration of Electron-Beam Inactivated Rhodococcus equi Failed to Protect Foals against Intrabronchial Infection with Live, Virulent R. equi." in: PLoS ONE, Vol. 11, Issue 2, pp. e0148111, (2016) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Gene ID: 100034181

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy