Interferon gamma antibody

Catalog No. ABIN6963774

The Mouse Monoclonal anti-Interferon gamma antibody has been validated for ELISA and ELISpot. It is suitable to detect Interferon gamma in samples from Cow and Sheep. There are 5+ publications available.

Quick Overview for Interferon gamma antibody (ABIN6963774)

Target: Interferon gamma (IFNG)

Reactivity: Cow, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: ELISA, ELISpot

Clone: MT17-1

Product Details anti-Interferon gamma Antibody

Specificity: Native and recombinant IFN-γ

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IFN-γ from sheep.

Characteristics: This monoclonal antibody enables specific detection of bovine IFN-γ in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant bovine IFN-gamma

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Is recommended as capture mAb in ELISpot, FluoroSpot, and ELISA in combination with detection mAb ABIN6963775, ABIN7448244, ABIN6963776 and ABIN6963777 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-Interferon gamma antibody (ABIN6963774)

Orbegozo-Medina, Martínez-Sernández, González-Warleta, Castro-Hermida, Mezo, Ubeira: "Vaccination of sheep with Quil-A® adjuvant expands the antibody repertoire to the Fasciola MF6p/FhHDM-1 antigen and administered together impair the growth and antigen release of flukes." in: Vaccine, Vol. 36, Issue 15, pp. 1949-1957, (2018) (PubMed).

Goosen, van Helden, Warren, Miller, Parsons: "The stability of plasma IP-10 enhances its utility for the diagnosis of Mycobacterium bovis infection in African buffaloes (Syncerus caffer)." in: Veterinary immunology and immunopathology, Vol. 173, pp. 17-20, (2016) (PubMed).

Rojas, Moreno, Valcárcel, Peña, Sevilla, Martín et al.: "Vaccination with recombinant adenoviruses expressing the peste des petits ruminants virus F or H proteins overcomes viral immunosuppression and induces protective immunity against PPRV challenge in ..." in: PLoS ONE, Vol. 9, Issue 7, pp. e101226, (2015) (PubMed).

Katz, Fargnoli, Williams, Steuerwald, Isidro, Ivanina, Sokolova, Bridges et al.: "Safety and efficacy of high-dose adeno-associated virus 9 encoding sarcoplasmic reticulum Ca(2+) adenosine triphosphatase delivered by molecular cardiac surgery with recirculating delivery in ovine ..." in: The Journal of thoracic and cardiovascular surgery, Vol. 148, Issue 3, pp. 1065-72, 1073e1-2; discussion1072-3, (2014) (PubMed).

Vordermeier, Dean, Rosenkrands, Agger, Andersen, Kaveh, Hewinson, Hogarth: "Adjuvants induce distinct immunological phenotypes in a bovine tuberculosis vaccine model." in: Clinical and vaccine immunology : CVI, Vol. 16, Issue 10, pp. 1443-8, (2009) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Gene ID: 281237, 443396

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy