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Interferon gamma antibody (Biotin)

This anti-Interferon gamma antibody is a Mouse Monoclonal antibody detecting Interferon gamma in ELISA, FACS and ELISpot. Suitable for Cow, Sheep and Goat. This Primary Antibody has been cited in 4+ publications.
Catalog No. ABIN6963775

Quick Overview for Interferon gamma antibody (Biotin) (ABIN6963775)

Target

See all Interferon gamma (IFNG) Antibodies
Interferon gamma (IFNG)

Reactivity

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Cow, Sheep, Goat

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Interferon gamma antibody is conjugated to Biotin

Application

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ELISA, Flow Cytometry (FACS), ELISpot

Clone

MT307
  • Specificity

    Native and recombinant IFN-γ

    Cross-Reactivity (Details)

    The monoclonal antibody cross-reacts with IFN-γ from sheep and goat.

    Characteristics

    This monoclonal antibody enables specific detection of bovine IFN-γ in immunoassays such as ELISpot, FluoroSpot, ELISA, and flow cytometry. Serum/Plasma samples

    Purification

    Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

    Sterility

    0.2 μm filtered

    Immunogen

    Recombinant bovine IFN-gamma

    Isotype

    IgG2a
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    ABIN6963775, ABIN7448244, ABIN6963776 and ABIN6963777 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb .1 . ABIN6963775, ABIN7448244, ABIN6963776 and ABIN6963777 is also recommended for use in flow cytometry.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

    Expiry Date

    18 months
  • Sajiki, Konnai, Okagawa, Nishimori, Maekawa, Goto, Ikebuchi, Nagata, Kawaji, Kagawa, Yamada, Kato, Nakajima, Suzuki, Murata, Mori, Ohashi: "Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease." in: Infection and immunity, Vol. 86, Issue 5, (2018) (PubMed).

    Orbegozo-Medina, Martínez-Sernández, González-Warleta, Castro-Hermida, Mezo, Ubeira: "Vaccination of sheep with Quil-A® adjuvant expands the antibody repertoire to the Fasciola MF6p/FhHDM-1 antigen and administered together impair the growth and antigen release of flukes." in: Vaccine, Vol. 36, Issue 15, pp. 1949-1957, (2018) (PubMed).

    Rojas, Moreno, Valcárcel, Peña, Sevilla, Martín et al.: "Vaccination with recombinant adenoviruses expressing the peste des petits ruminants virus F or H proteins overcomes viral immunosuppression and induces protective immunity against PPRV challenge in ..." in: PLoS ONE, Vol. 9, Issue 7, pp. e101226, (2015) (PubMed).

    Vordermeier, Dean, Rosenkrands, Agger, Andersen, Kaveh, Hewinson, Hogarth: "Adjuvants induce distinct immunological phenotypes in a bovine tuberculosis vaccine model." in: Clinical and vaccine immunology : CVI, Vol. 16, Issue 10, pp. 1443-8, (2009) (PubMed).

  • Target

    Interferon gamma (IFNG)

    Alternative Name

    IFNG

    Gene ID

    281237, 443396

    Pathways

    Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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