Interferon gamma antibody (FITC)

Catalog No. ABIN6963776

This Mouse Monoclonal antibody specifically detects Interferon gamma in FACS. It exhibits reactivity toward Cow, Sheep and Goat.

Quick Overview for Interferon gamma antibody (FITC) (ABIN6963776)

Target: Interferon gamma (IFNG)

Reactivity: Cow, Sheep, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: MT307

Product Details anti-Interferon gamma Antibody (FITC)

Specificity: Native and recombinant IFN-γ.

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IFN-γ from sheep and goat.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant bovine IFN-gamma

Isotype: IgG2a

Application Details

Application Notes: Detection of IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IFN-γ by flow cytometry in bovine peripheral blood mononuclear cells (PBMC). Bovine PBMC were stimulated with PMA/ionomycin for 16 hours in the presence of Brefeldin A. The cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with anti-bovine IFN-γ mAb MT307-FITC (right panel). The left panel depicts stimulated cells stained with a matched isotype control antibody. The dot plots derive from a lymphocyte gate. Flow cytometry was performed on a Guava EasyCyte Mini.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Expiry Date: 18 months

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Gene ID: 281237, 443396

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy