Interferon gamma antibody (PromoFluor 647 Premium)

Catalog No. ABIN6963777

The Mouse Monoclonal anti-Interferon gamma antibody has been validated for FACS. It is suitable to detect Interferon gamma in samples from Cow, Sheep and Goat. There is 1 publication available.

Quick Overview for Interferon gamma antibody (PromoFluor 647 Premium) (ABIN6963777)

Target: Interferon gamma (IFNG)

Reactivity: Cow, Sheep, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is conjugated to PromoFluor 647 Premium

Application: Flow Cytometry (FACS)

Clone: MT307

Product Details anti-Interferon gamma Antibody (PromoFluor 647 Premium)

Specificity: Native and recombinant IFN-γ.

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IFN-γ from sheep and goat.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant bovine IFN-gamma

Isotype: IgG2a

Application Details

Application Notes: Detection of IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IFN-γ by flow cytometry in viable bovine PBMC. Cells were stimulated for 16 hours in the presence of PHA and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with MT307-PF647P (left panel). The specificity of MT307-PF647P was demonstrated by pre-incubating the antibody with recombinant bovine IFN-γ prior to staining of PBMC (middle panel). Matched isotype control antibody (right panel). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Expiry Date: 18 months

References for anti-Interferon gamma antibody (PromoFluor 647 Premium) (ABIN6963777)

Richt, Kasinathan, Hamir, Castilla, Sathiyaseelan, Vargas, Sathiyaseelan, Wu, Matsushita, Koster, Kato, Ishida, Soto, Robl, Kuroiwa: "Production of cattle lacking prion protein." in: Nature biotechnology, Vol. 25, Issue 1, pp. 132-8, (2007) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Gene ID: 281237, 443396

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy