Interferon gamma antibody (PromoFluor 647 Premium)

Catalog No. ABIN6963785

This Mouse Monoclonal antibody specifically detects Interferon gamma in FACS. It exhibits reactivity toward Guinea Pig.

Quick Overview for Interferon gamma antibody (PromoFluor 647 Premium) (ABIN6963785)

Target: Interferon gamma (IFNG)

Reactivity: Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is conjugated to PromoFluor 647 Premium

Application: Flow Cytometry (FACS)

Clone: MT4A27

Product Details anti-Interferon gamma Antibody (PromoFluor 647 Premium)

Specificity: Native and recombinant IFN-γ

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant guinea pig IFN-gamma

Isotype: IgG1

Application Details

Application Notes: Detection of guinea pig IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. PF647P is excited by the red laser (633 nm). The excitation max is 654 nm and the emission max is 672 nm. Detection of IFN-γ by flow cytometry in viable guinea pig spleen cells. Cells were stimulated for 16 hrs in the presence of PMA/ ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with MT4A27-PF647P (solid line). Matched isotype control antibody (dashed line). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Expiry Date: 18 months

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Gene ID: 100379568

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy