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Interferon gamma antibody (Biotin)

The Mouse Monoclonal anti-Interferon gamma antibody has been validated for ELISA, FACS and ELISpot. It is suitable to detect Interferon gamma in samples from Pig. There are 5+ publications available.
Catalog No. ABIN6963789

Quick Overview for Interferon gamma antibody (Biotin) (ABIN6963789)

Target

See all Interferon gamma (IFNG) Antibodies
Interferon gamma (IFNG)

Reactivity

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Pig

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Interferon gamma antibody is conjugated to Biotin

Application

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ELISA, Flow Cytometry (FACS), ELISpot

Clone

P2C11
  • Specificity

    Native and recombinant porcine IFN-γ

    Characteristics

    This monoclonal antibody enables sensitive and specific detection of porcine IFN-γ immunoassays such as ELISpot, FluoroSpot, ELISA, and flow cytometry. Serum/Plasma samples

    Purification

    Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

    Sterility

    0.2 μm filtered

    Immunogen

    Recombinant porcine IFN-gamma

    Isotype

    IgG2a
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    ABIN6963789, ABIN7448252, ABIN6963790 and ABIN6963791 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb pIFNγ-I . ABIN6963789, ABIN7448252, ABIN6963790 and ABIN6963791 is also recommended for use in flow cytometry.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

    Expiry Date

    18 months
  • Stadler, Naderer, Beffort, Ritzmann, Emrich, Hermanns, Fiebig, Saalmüller, Gerner, Glatthaar-Saalmüller, Ladinig: "Safety and immune responses after intradermal application of Porcilis PRRS in either the neck or the perianal region." in: PLoS ONE, Vol. 13, Issue 9, pp. e0203560, (2019) (PubMed).

    Suárez, Sordo, Prieto, Rodríguez, Méndez, Rodríguez, Rodríguez-Mallon, Lorenzo, Santana, González, Naranjo, Frías, Carpio, Estrada: "A single dose of the novel chimeric subunit vaccine E2-CD154 confers early full protection against classical swine fever virus." in: Vaccine, Vol. 35, Issue 34, pp. 4437-4443, (2018) (PubMed).

    Jeong, Kang, Kim, Park, Park, Oh, Yang, Chae: "A modified-live porcine reproductive and respiratory syndrome virus (PRRSV)-1 vaccine protects late-term pregnancy gilts against heterologous PRRSV-1 but not PRRSV-2 challenge." in: Transboundary and emerging diseases, Vol. 65, Issue 5, pp. 1227-1234, (2018) (PubMed).

    Lopera-Madrid, Osorio, He, Xiang, Adams, Laughlin, Mwangi, Subramanya, Neilan, Brake, Burrage, Brown, Clavijo, Bounpheng: "Safety and immunogenicity of mammalian cell derived and Modified Vaccinia Ankara vectored African swine fever subunit antigens in swine." in: Veterinary immunology and immunopathology, Vol. 185, pp. 20-33, (2017) (PubMed).

    Sedlak, Patzl, Saalmüller, Gerner: "IL-12 and IL-18 induce interferon-γ production and de novo CD2 expression in porcine γδ T cells." in: Developmental and comparative immunology, Vol. 47, Issue 1, pp. 115-22, (2015) (PubMed).

  • Target

    Interferon gamma (IFNG)

    Alternative Name

    IFNG

    Gene ID

    396991

    Pathways

    Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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