Interferon gamma antibody (PromoFluor 647 Premium)

Catalog No. ABIN6963791

This Mouse Monoclonal antibody specifically detects Interferon gamma in FACS. It exhibits reactivity toward Pig.

Quick Overview for Interferon gamma antibody (PromoFluor 647 Premium) (ABIN6963791)

Target: Interferon gamma (IFNG)

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is conjugated to PromoFluor 647 Premium

Application: Flow Cytometry (FACS)

Clone: P2C11

Product Details anti-Interferon gamma Antibody (PromoFluor 647 Premium)

Specificity: Native and recombinant porcine IFN-γ

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant porcine IFN-gamma

Isotype: IgG2a

Application Details

Application Notes: Detection of IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IFN-γ by flow cytometry in viable porcine PBMC. Cells were stimulated for 16 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with CD3-PE and P2C11-PF647P (right panel). The left panel depicts cells stained with a matched isotype control antibody. The dot plots derive from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Expiry Date: 18 months

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Gene ID: 396991

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy