Interferon gamma antibody (Biotin)

Catalog No. ABIN6963816

The Rat Monoclonal anti-Interferon gamma antibody has been validated for ELISA and ELISpot. It is suitable to detect Interferon gamma in samples from Mouse. There are 4+ publications available.

Quick Overview for Interferon gamma antibody (Biotin) (ABIN6963816)

Target: Interferon gamma (IFNG)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is conjugated to Biotin

Application: ELISA, ELISpot

Clone: R4-6A2

Product Details anti-Interferon gamma Antibody (Biotin)

Specificity: Native and recombinant mouse IFN-γ

Characteristics: This monoclonal antibody enables specific detection of mouse IFN-γ in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Native mouse IFN-gamma

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963816, ABIN7448269 and ABIN6964014 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb ABIN6963815 and ABIN7448268 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-Interferon gamma antibody (Biotin) (ABIN6963816)

Bourgeois-Daigneault, Roy, Aitken, El Sayes, Martin, Varette, Falls, St-Germain, Pelin, Lichty, Stojdl, Ungerechts, Diallo, Bell: "Neoadjuvant oncolytic virotherapy before surgery sensitizes triple-negative breast cancer to immune checkpoint therapy." in: Science translational medicine, Vol. 10, Issue 422, (2019) (PubMed).

Veglia, Tyurin, Blasi, De Leo, Kossenkov, Donthireddy, To, Schug, Basu, Wang, Ricciotti, DiRusso, Murphy, Vonderheide, Lieberman, Mulligan, Nam, Hockstein, Masters, Guarino, Lin, Nefedova, Black et al.: "Fatty acid transport protein 2 reprograms neutrophils in cancer. ..." in: Nature, Vol. 569, Issue 7754, pp. 73-78, (2019) (PubMed).

Noguera-Ortega, Blanco-Cabra, Rabanal, Sánchez-Chardi, Roldán, Guallar-Garrido, Torrents, Luquin, Julián: "Mycobacteria emulsified in olive oil-in-water trigger a robust immune response in bladder cancer treatment." in: Scientific reports, Vol. 6, pp. 27232, (2018) (PubMed).

Gregor, Wolchok, Ferrone, Buchinshky, Guevara-Patiño, Perales, Mortazavi, Bacich, Heston, Latouche, Sadelain, Allison, Scher, Houghton: "CTLA-4 blockade in combination with xenogeneic DNA vaccines enhances T-cell responses, tumor immunity and autoimmunity to self antigens in animal and cellular model systems." in: Vaccine, Vol. 22, Issue 13-14, pp. 1700-8, (2004) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Gene ID: 15978

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy