Interferon gamma antibody (Biotin)

Catalog No. ABIN6963838

This anti-Interferon gamma antibody is a Mouse Monoclonal antibody detecting Interferon gamma in ELISA and ELISpot. Suitable for Human and Non-Human Primate. This Primary Antibody has been cited in 4+ publications.

Quick Overview for Interferon gamma antibody (Biotin) (ABIN6963838)

Target: Interferon gamma (IFNG)

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is conjugated to Biotin

Application: ELISA, ELISpot

Clone: 7-B6-1

Product Details anti-Interferon gamma Antibody (Biotin)

Specificity: Native and recombinant IFN-γ

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IFN-γ from non-human primates.

Characteristics: This monoclonal antibody enables specific detection of human IFN-γ in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human IFN-gamma

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963838, ABIN7448287, ABIN6963840 and ABIN6963841 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb ABIN6963837, ABIN7448286 and ABIN6963839 for human IFN-γ .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-Interferon gamma antibody (Biotin) (ABIN6963838)

Sharpe, Smyth, McIntyre, Gleeson, Dennis: "Refinement and reduction through application of a quantitative score system for estimation of TB-induced disease burden using computed tomography." in: Laboratory animals, Vol. 52, Issue 6, pp. 599-610, (2019) (PubMed).

Wentink, Verheul, Griffioen, Schafer, McPherson, Early, van der Vliet, de Gruijl: "A safety and immunogenicity study of immunization with hVEGF26-104/RFASE in cynomolgus monkeys." in: Vaccine, Vol. 36, Issue 15, pp. 2025-2032, (2018) (PubMed).

Sahin, Derhovanessian, Miller, Kloke, Simon, Löwer, Bukur, Tadmor, Luxemburger, Schrörs, Omokoko, Vormehr, Albrecht, Paruzynski, Kuhn, Buck, Heesch, Schreeb, Müller, Ortseifer, Vogler, Godehardt et al.: "Personalized RNA mutanome vaccines mobilize poly-specific therapeutic immunity against cancer. ..." in: Nature, Vol. 547, Issue 7662, pp. 222-226, (2017) (PubMed).

Arakawa, Siewert, Stöhr, Besgen, Kim, Rühl, Nickel, Vollmer, Thomas, Krebs, Pinkert, Spannagl, Held, Kammerbauer, Besch, Dornmair, Prinz: "Melanocyte antigen triggers autoimmunity in human psoriasis." in: The Journal of experimental medicine, Vol. 212, Issue 13, pp. 2203-12, (2016) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Gene ID: 3458

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy