IFNA Pan antibody (Biotin)

Catalog No. ABIN6963846

Product Details

Target: IFNA Pan

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, ELISpot

Specificity: Native and recombinant IFN-α

Cross-Reactivity (Details): The monoclonal antibodies cross-react with IFN-α from non-human primates.

Characteristics: These monoclonal antibodies enable specific detection of human IFN-α subtypes 1/13, 2, 4, 5, 6, 7, 8, 10, 14, 16 and 17 (pan-specific detection) in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human IFN-alpha

Clone: MT2-4-6

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963844 and ABIN7448290/4/6 are recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb ABIN6963843 and ABIN7448289/3/5 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References

Bao, Wang, Liu, Shi, Lu, Sha, Weng, Hanabuchi, Qin, Plumas, Chaperot, Zhang, Liu: "NFATC3 promotes IRF7 transcriptional activity in plasmacy--toid dendritic cells." in: The Journal of experimental medicine, Vol. 213, Issue 11, pp. 2383-2398, (2017) (PubMed).

Dudhgaonkar, Ranade, Nagar, Subramani, Prasad, Karunanithi, Srivastava, Venkatesh, Selvam, Krishnamurthy, Mariappan, Saxena, Fan, Stetsko, Holloway, Li, Zhu, Yang, Ruepp, Nair, Santella, Duncia et al.: "Selective IRAK4 Inhibition Attenuates Disease in Murine Lupus Models and Demonstrates Steroid Sparing Activity. ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 3, pp. 1308-1319, (2017) (PubMed).

Crisci, Ellegård, Nyström, Rondahl, Serrander, Bergström, Sjöwall, Eriksson, Larsson: "Complement Opsonization Promotes Herpes Simplex Virus 2 Infection of Human Dendritic Cells." in: Journal of virology, Vol. 90, Issue 10, pp. 4939-4950, (2017) (PubMed).

Moody, Santra, Vandergrift, Sutherland, Gurley, Drinker, Allen, Xia, Meyerhoff, Parks, Lloyd, Easterhoff, Alam, Liao, Ward, Ferrari, Montefiori, Tomaras, Seder, Letvin, Haynes: "Toll-like receptor 7/8 (TLR7/8) and TLR9 agonists cooperate to enhance HIV-1 envelope antibody responses in rhesus macaques." in: Journal of virology, Vol. 88, Issue 6, pp. 3329-39, (2014) (PubMed).

Vallhov, Qin, Johansson, Ahlborg, Muhammed, Scheynius, Gabrielsson: "The importance of an endotoxin-free environment during the production of nanoparticles used in medical applications." in: Nano letters, Vol. 6, Issue 8, pp. 1682-6, (2006) (PubMed).

Target Details

Target: IFNA Pan

Gene ID: 3439, 3440, 3441