IL-10 antibody (Biotin)

Catalog No. ABIN6963848

This Rat Monoclonal antibody specifically detects IL-10 in ELISA and ELISpot. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for IL-10 antibody (Biotin) (ABIN6963848)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-10 antibody is conjugated to Biotin

Application: ELISA, ELISpot

Clone: 12G8

Product Details anti-IL-10 Antibody (Biotin)

Specificity: Native and recombinant human IL-10

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human IL-10

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL-10 antibody (Biotin) (ABIN6963848)

Järver, Dondalska, Poux, Sandberg, Bergenstråhle, Sköld, Dereuddre-Bosquet, Martinon, Pålsson, Zaghloul, Brodin, Sander, Lennox, Behlke, El-Andaloussi, Lehtiö, Lundeberg, LeGrand, Spetz: "Single-Stranded Nucleic Acids Regulate TLR3/4/7 Activation through Interference with Clathrin-Mediated Endocytosis." in: Scientific reports, Vol. 8, Issue 1, pp. 15841, (2019) (PubMed).

Tagawa, Albanese, Bouvet, Moosmann, Mautner, Heissmeyer, Zielinski, Lutter, Hoser, Hastreiter, Hayes, Sugden, Hammerschmidt: "Epstein-Barr viral miRNAs inhibit antiviral CD4+ T cell responses targeting IL-12 and peptide processing." in: The Journal of experimental medicine, Vol. 213, Issue 10, pp. 2065-80, (2017) (PubMed).

Naouar, Boussoffara, Chenik, Gritli, Ben Ahmed, Belhadj Hmida, Bahi-Jaber, Bardi, Gorgi, Ben Salah, Louzir: "Prediction of T Cell Epitopes from Leishmania major Potentially Excreted/Secreted Proteins Inducing Granzyme B Production." in: PLoS ONE, Vol. 11, Issue 1, pp. e0147076, (2016) (PubMed).

Johansson, Saghafian-Hedengren, Haileselassie, Roos, Troye-Blomberg, Nilsson, Sverremark-Ekström: "Early-life gut bacteria associate with IL-4-, IL-10- and IFN-γ production at two years of age." in: PLoS ONE, Vol. 7, Issue 11, pp. e49315, (2013) (PubMed).

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL10

Gene ID: 3586

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints