GM-CSF antibody

Catalog No. ABIN6963888

The Rat Monoclonal anti-GM-CSF antibody has been validated for ELISA and ELISpot. It is suitable to detect GM-CSF in samples from Human and Non-Human Primate. There are 5+ publications available.

Quick Overview for GM-CSF antibody (ABIN6963888)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Human, Non-Human Primate

Host: Rat

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is un-conjugated

Application: ELISA, ELISpot

Clone: 21C11

Product Details anti-GM-CSF Antibody

Specificity: Native and recombinant GM-CSF

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with GM-CSF from non-human primates.

Characteristics: This monoclonal antibody enables specific detection of human GM-CSF in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human GM-CSF

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963888 and ABIN7489151 is recommended as capture mAb in ELISpot, FluoroSpot, and ELISA in combination with detection mAb ABIN6963889, ABIN7489152 and ABIN6964026 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-GM-CSF antibody (ABIN6963888)

Jarrett, Salio, Lloyd-Lavery, Subramaniam, Bourgeois, Archer, Cheung, Hardman, Chandler, Salimi, Gutowska-Owsiak, de la Serna, Fallon, Jolin, Mckenzie, Dziembowski, Podobas, Bal, Johnson, Moody et al.: "Filaggrin inhibits generation of CD1a neolipid antigens by house dust mite-derived phospholipase. ..." in: Science translational medicine, Vol. 8, Issue 325, pp. 325ra18, (2016) (PubMed).

Myers, Cooper, Kem, Stavrakis, Kosanke, Shevach, Fairweather, Stoner, Cox, Cunningham: "Cardiac myosin-Th17 responses promote heart failure in human myocarditis." in: JCI insight, Vol. 1, Issue 9, (2016) (PubMed).

Morita, Yamamoto, Suzuki, Mori, Igarashi, Sugita: "Molecular requirements for T cell recognition of N-myristoylated peptides derived from the simian immunodeficiency virus Nef protein." in: Journal of virology, Vol. 87, Issue 1, pp. 482-8, (2013) (PubMed).

Gille-Johnson, Smedman, Gudmundsdotter, Somell, Nihlmark, Paulie, Andersson, Gårdlund: "Circulating monocytes are not the major source of plasma cytokines in patients with sepsis." in: Shock (Augusta, Ga.), Vol. 38, Issue 6, pp. 577-83, (2013) (PubMed).

Mäkitalo, Andersson, Areström, Karlén, Villinger, Ansari, Paulie, Thorstensson, Ahlborg: "ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques." in: Journal of immunological methods, Vol. 270, Issue 1, pp. 85-97, (2002) (PubMed).

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GMCSF

Gene ID: 1437

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin