GM-CSF antibody (Biotin)

Catalog No. ABIN6963889

This anti-GM-CSF antibody is a Rat Monoclonal antibody detecting GM-CSF in ELISA and ELISpot. Suitable for Human and Non-Human Primate. This Primary Antibody has been cited in 4+ publications.

Quick Overview for GM-CSF antibody (Biotin) (ABIN6963889)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Human, Non-Human Primate

Host: Rat

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is conjugated to Biotin

Application: ELISA, ELISpot

Clone: 23B6

Product Details anti-GM-CSF Antibody (Biotin)

Specificity: Native and recombinant GM-CSF

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with GM-CSF from non-human primates.

Characteristics: This monoclonal antibody enables specific detection of human GM-CSF in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human GM-CSF

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963889, ABIN7489152 and ABIN6964026 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb ABIN6963888 and ABIN7489151 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-GM-CSF antibody (Biotin) (ABIN6963889)

Ammitzbøll, Börnsen, Romme Christensen, Ratzer, Romme Nielsen, Søndergaard, von Essen, Sellebjerg: "Smoking reduces circulating CD26hiCD161hi MAIT cells in healthy individuals and patients with multiple sclerosis." in: Journal of leukocyte biology, Vol. 101, Issue 5, pp. 1211-1220, (2017) (PubMed).

Myers, Cooper, Kem, Stavrakis, Kosanke, Shevach, Fairweather, Stoner, Cox, Cunningham: "Cardiac myosin-Th17 responses promote heart failure in human myocarditis." in: JCI insight, Vol. 1, Issue 9, (2016) (PubMed).

Morita, Yamamoto, Suzuki, Mori, Igarashi, Sugita: "Molecular requirements for T cell recognition of N-myristoylated peptides derived from the simian immunodeficiency virus Nef protein." in: Journal of virology, Vol. 87, Issue 1, pp. 482-8, (2013) (PubMed).

Ruiss, Jochum, Wanner, Reisbach, Hammerschmidt, Zeidler: "A virus-like particle-based Epstein-Barr virus vaccine." in: Journal of virology, Vol. 85, Issue 24, pp. 13105-13, (2012) (PubMed).

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GMCSF

Gene ID: 1437

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin