Interleukin 17a antibody (FITC)

Catalog No. ABIN6963909

The Mouse Monoclonal anti-Interleukin 17a antibody has been validated for FACS. It is suitable to detect Interleukin 17a in samples from Human, Cow, Pig, Dog, Sheep, Horse and Non-Human Primate.

Quick Overview for Interleukin 17a antibody (FITC) (ABIN6963909)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivity: Human, Cow, Pig, Dog, Sheep, Horse, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interleukin 17a antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: MT504

Product Details anti-Interleukin 17a Antibody (FITC)

Specificity: Native and recombinant IL-17A.

Cross-Reactivity (Details): The antibody also cross-reacts with IL-17A from cow, sheep, horse, pig and dog.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant human IL-17A

Isotype: IgG1

Application Details

Application Notes: Detection of IL-17A producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-17A by flow cytometry in human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated with PMA/ionomycin for 16 hours in the presence of Brefeldin A. The cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with anti-human IL-17A mAb MT504-FITC and anti-human IFN-γ-PE (right panel). The left panel depicts stimulated cells stained with matched isotype control antibodies. The dot plots derive from a lymphocyte gate. Flow cytometry was performed on a BD FACSVerse system.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Expiry Date: 18 months

Target Details for Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: IL17A

Gene ID: 3605, 282863, 100034142