APOA1 antibody (Biotin)

Catalog No. ABIN6963953

This Rat Monoclonal antibody specifically detects APOA1 in WB and ELISA. It exhibits reactivity toward Mouse and has been mentioned in 2+ publications.

Quick Overview for APOA1 antibody (Biotin) (ABIN6963953)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This APOA1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Clone: MHDL36

Product Details anti-APOA1 Antibody (Biotin)

Specificity: Native apolipoprotein A1 (apoA1)

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Purified mouse apoA1

Isotype: IgG2a

Application Details

Application Notes: For quantification of apoA1 in solution e.g. serum/plasma samples and cell culture supernatants using ELISA. mHDL36 is recommended as detection mAb in combination with coating mAb mHDL93 . The antibody is also suitable for Western blot.

Comment:

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-APOA1 antibody (Biotin) (ABIN6963953)

Mishra, Muthuramu, Aboumsallem, Kempen, De Geest: "Reconstituted HDL (Milano) Treatment Efficaciously Reverses Heart Failure with Preserved Ejection Fraction in Mice." in: International journal of molecular sciences, Vol. 19, Issue 11, (2019) (PubMed).

Aboumsallem, Mishra, Amin, Muthuramu, Kempen, De Geest: "Successful treatment of established heart failure in mice with recombinant HDL (Milano)." in: British journal of pharmacology, Vol. 175, Issue 21, pp. 4167-4182, (2019) (PubMed).

Target Details for APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: apoA1

Gene ID: 11806

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism