Interleukin 17a antibody

Catalog No. ABIN6964028

This Mouse Monoclonal antibody specifically detects Interleukin 17a in Neut. It exhibits reactivity toward Human.

Quick Overview for Interleukin 17a antibody (ABIN6964028)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interleukin 17a antibody is un-conjugated

Application: Neutralization (Neut)

Clone: MT504

Product Details anti-Interleukin 17a Antibody

Specificity: Native and recombinant IL-17A.

Cross-Reactivity (Details): The antibody cross-reacts with IL-17A from non-human primates. Cross-reactivity has been tested in ELISA and/or ELISpot.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant human IL-17A

Isotype: IgG1

Application Details

Application Notes: Neutralization of human IL-17A bioactivity. It is recommended to establish the optimal antibody concentration for the assay system used. The antibody is also suitable for immunoassays. Neutralization Neutralization assay: Neutralization of human IL-17A induced IL-6 production (see graph). To determine the neutralizing capacity and ND50, various concentrations of mAb MT504 and human recombinant IL-17A were added to normal human dermal fibroblasts. Following overnight incubation, cell supernatants were quantified in IL-6 ELISA. Neutralization dose 50 (ND50): MT504 neutralized the effect of IL-17A at a ND50value of 0.01-0.03 μg/mL when 10 ng/mL IL-17A was present. ND50 is defined as the concentration of antibody that results in 50 % inhibition of the biological response under the experimental conditions specified.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS free of preservatives

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Expiry Date: 18 months

Target Details for Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: IL17A

Gene ID: 3605