CD3 antibody

Catalog No. ABIN6964040

This Mouse Monoclonal antibody specifically detects CD3 in St. It exhibits reactivity toward Non-Human Primate.

Quick Overview for CD3 antibody (ABIN6964040)

Target: CD3

Reactivity: Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Stimulation (St)

Clone: CD3-1

Product Details anti-CD3 Antibody

Specificity: Recognizes human CD3 and CD3 from rhesus and cynomolgus macaques.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Endotoxin Level: The endotoxin content is below 1 EU/mg.

Immunogen: Stimulated human leukocytes

Isotype: IgG3

Application Details

Application Notes: The anti-CD3 mAb is a useful tool for polyclonal activation of T cells. It can be added in solution or adsorbed to a solid phase. It is suitable for stimulation of T cells to induce secretion of IFN-γ in the ELISpot assay. It can also be used for induction of other T-cell cytokines, e.g. IL-2, IL-4, IL-13, IL-17A and perforin. The recommended concentration for cytokine secretion is 100 ng/mL but should be titrated.

Restrictions: For Research Use only

Handling

Buffer: supplied at 100 μg/mL in PBS free of preservatives

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing. The expiry date indicates how long unopened products, stored according to instructions, are recommended for use.

Expiry Date: 18 months

Target Details for CD3

Target: CD3

Alternative Name: CD3

Gene ID: 916

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway