AGO2 antibody (AA 1-148)

Catalog No. ABIN6971363

This Mouse Monoclonal antibody specifically detects AGO2 in WB, IF, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for AGO2 antibody (AA 1-148) (ABIN6971363)

Target: AGO2 (Argonaute 2 (AGO2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AGO2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 1B1-E2H5

Product Details anti-AGO2 Antibody

Binding Specificity: AA 1-148

Characteristics: Argonaute 2 (Ago2, aka EIF2C2) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage. Ago2 antibody (mAb) (Clone 1B1-E2H5) was raised in a Mouse host. It has been validated for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western blot, it has been shown to react with Human samples.

Purification: Protein G Chromatography

Immunogen: This Ago2 antibody was raised against a recombinant protein corresponding to amino acids 1-148 of human Ago2.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for AGO2

Target: AGO2 (Argonaute 2 (AGO2))

Alternative Name: Ago2

Molecular Weight: 100 kDa

NCBI Accession: NP_036286

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization