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AGO2 antibody (AA 1-148)

This Mouse Monoclonal antibody specifically detects AGO2 in WB, IF, IP and ICC. It exhibits reactivity toward Human.
Catalog No. ABIN6971363

Quick Overview for AGO2 antibody (AA 1-148) (ABIN6971363)

Target

See all AGO2 Antibodies
AGO2 (Argonaute 2 (AGO2))

Reactivity

  • 47
  • 24
  • 15
  • 1
  • 1
  • 1
  • 1
Human

Host

  • 46
  • 3
Mouse

Clonality

  • 32
  • 17
Monoclonal

Conjugate

  • 26
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
This AGO2 antibody is un-conjugated

Application

  • 28
  • 20
  • 17
  • 12
  • 7
  • 5
  • 5
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone

1B1-E2H5
  • Binding Specificity

    • 8
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-148

    Characteristics

    Argonaute 2 (Ago2, aka EIF2C2) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage. Ago2 antibody (mAb) (Clone 1B1-E2H5) was raised in a Mouse host. It has been validated for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western blot, it has been shown to react with Human samples.

    Purification

    Protein G Chromatography

    Immunogen

    This Ago2 antibody was raised against a recombinant protein corresponding to amino acids 1-148 of human Ago2.

    Isotype

    IgG2a
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Buffer

    Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
  • Target

    AGO2 (Argonaute 2 (AGO2))

    Alternative Name

    Ago2

    Molecular Weight

    100 kDa

    NCBI Accession

    NP_036286

    Pathways

    Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization
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