AGO4 antibody (AA 1-164)

Catalog No. ABIN6971367

The Mouse Monoclonal anti-AGO4 antibody has been validated for WB and IP. It is suitable to detect AGO4 in samples from Human.

Quick Overview for AGO4 antibody (AA 1-164) (ABIN6971367)

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AGO4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 1B7-G11

Product Details anti-AGO4 Antibody

Binding Specificity: AA 1-164

Characteristics: Argonaute 4 (Ago4, aka EIF2C4) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Ago4 lacks RNase activity and does not appear to cleave target mRNA molecules. It binds to microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Ago4 has both a PAZ and a PIWI domain, indicative of the Argonaute family of proteins. Ago4 antibody (mAb) (Clone 1B7-G11) was raised in a Mouse host. It has been validated for use in Immunoprecipitation and Western blot, it has been shown to react with Human samples.

Purification: Protein G Chromatography

Immunogen: This Ago4 antibody was raised against a recombinant protein corresponding to amino acids 1-164 of human Ago4.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for AGO4

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Alternative Name: Ago4

Molecular Weight: 100 kDa

NCBI Accession: NP_060099

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Cellular Glucan Metabolic Process