BUB1B antibody (AA 1-350)

Catalog No. ABIN6971476

This anti-BUB1B antibody is a Mouse Monoclonal antibody detecting BUB1B in IF and ICC. Suitable for Human and Mouse.

Quick Overview for BUB1B antibody (AA 1-350) (ABIN6971476)

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BUB1B antibody is un-conjugated

Application: Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 8G1

Product Details anti-BUB1B Antibody

Binding Specificity: AA 1-350

Characteristics: BubR1 (also known as BUB1B, MAD3L and SSK1) is an essential component of the mitotic spindle integrity checkpoint and is required for the normal progression of mitosis, and thus can act as a tumor suppressor. It encodes a serine / threonine kinase localized to the kinetochore and is involved in spindle checkpoint function. BubR1 plays a role in the inhibition of the anaphase-promoting complex (APC), delaying the onset of anaphase and ensuring proper chromosome segregation. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle and the chromosomes are aligned on the metaphase plate. Another function involves the monitoring of kinetochore activities that depend on the kinetochore motor CENP-E. It is also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. BubR1 is autophosphorylated, and BubR1 kinase activity is stimulated by association with the centromere protein CENP-E. Phosphorylation at Ser670 and Ser1043 occurs at kinetochores upon mitotic entry with dephosphorylation occurring at the onset of anaphase. Impaired spindle checkpoint function has been found in many forms of cancer. Defects in BubR1 are involved in mosaic variegated aneuploidy syndrome (MVA) and premature chromatid separation trait (PCS). BubR1 interacts with CENP-E, CENP-F, mitosin, PLK1, CASC5, BUB3 and the anaphase-promoting complex (APC). It is part of a complex containing BUB3 and CDC2. BubR1 antibody (mAb) (Clone 8G1) was raised in a Mouse host. It has been validated for use in Immunocytochemistry and Immunofluorescence, it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.

Purification: Protein G Chromatography

Immunogen: This BubR1 antibody was raised against recombinant protein corresponding to amino acids 1-350 of human BubR1.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Alternative Name: BubR1

Molecular Weight: 130 kDa

NCBI Accession: NP_001202