CENPA antibody (pSer16, pSer18)

Catalog No. ABIN6971521

This anti-CENPA antibody is a Rabbit Polyclonal antibody detecting CENPA in WB, IF, ICC and DB. Suitable for Human.

Quick Overview for CENPA antibody (pSer16, pSer18) (ABIN6971521)

Target: CENPA (Centromere Protein A (CENPA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB)

Product Details anti-CENPA Antibody

Binding Specificity: pSer16, pSer18

Characteristics: CENP-A (Centromere Protein A) is a Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. Recently, phosphorylation of CENP-A on Ser16 and Ser18 was reported and found to be important for chromosome segregation during mitosis. CENP-A phospho Ser18 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Affinity Purified

Immunogen: This antibody was raised against a synthetic peptide containing phosphorylated serine 16 and 18 of human CENP-A.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for CENPA

Target: CENPA (Centromere Protein A (CENPA))

Alternative Name: CENP-A

Molecular Weight: 16 kDa

NCBI Accession: NP_001800

Pathways: Chromatin Binding, Maintenance of Protein Location