DDB1 antibody (C-Term)

Catalog No. ABIN6971574

The Rabbit Polyclonal anti-DDB1 antibody has been validated for WB. It is suitable to detect DDB1 in samples from Human.

Quick Overview for DDB1 antibody (C-Term) (ABIN6971574)

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDB1 Antibody

Binding Specificity: C-Term

Characteristics: DDB1 (DNA damage-binding protein 1, HBV X-associated protein 1, XAP-1, Xeroderma pigmentosum group E-complementing protein) is a DNA binding protein that associates with DNA following UV damage and functions in excision repair. In addition to its function in DNA repair, DDB1 plays a role in normal mitotic exit by regulating Anaphase Promoting Complex (APC) activity. Depletion of DDB1 in mitotic cells significantly delays mitotic exit. DDB1 facilitates the binding of substrates to the Cul4-Roc1 complex and the ubiquitination of selected proteins, including histones H2A, H3 and H4 localized to the sites of DNA damage. It is also involved in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of P53 in response to radiation-induced DNA damage and during DNA replication. In mouse studies, it was identified that loss of DDB1 function prevents hepatocytes from replicating DNA, inducing compensatory proliferation of normal hepatocytes that leads to development of hepatocellular carcinoma. DDB1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot, it has been shown to react with Human samples.

Purification: Affinity Purified

Immunogen: This DDB1 antibody was raised against a peptide from the C-terminus of human DDB1.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for DDB1

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Alternative Name: DDB1

Molecular Weight: 140 kDa

NCBI Accession: NP_001914

Pathways: DNA Damage Repair