EZH1 antibody (N-Term)

Catalog No. ABIN6971640

The Rabbit Polyclonal anti-EZH1 antibody has been validated for WB. It is suitable to detect EZH1 in samples from Mouse.

Quick Overview for EZH1 antibody (N-Term) (ABIN6971640)

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EZH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EZH1 Antibody

Binding Specificity: N-Term

Characteristics: EZH1 (Enhancer Of Zeste Homolog 1 ) is a Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. EZH1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot, it has been shown to react with Mouse samples.

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide within the N-terminal region of human EZH1.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for EZH1

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Alternative Name: EZH1

Molecular Weight: 100 kDa

NCBI Accession: NP_001982