H2AFV antibody (C-Term)

Catalog No. ABIN6971793

The Mouse Monoclonal anti-H2AFV antibody has been validated for WB and ChIP. It is suitable to detect H2AFV in samples from Drosophila melanogaster.

Quick Overview for H2AFV antibody (C-Term) (ABIN6971793)

Target: H2AFV (H2A Histone Family, Member V (H2AFV))

Reactivity: Drosophila melanogaster

Host: Mouse

Clonality: Monoclonal

Conjugate: This H2AFV antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Clone: 10E9-D1

Product Details anti-H2AFV Antibody

Binding Specificity: C-Term

Characteristics: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle.In Drosophila, the H2A variant corresponding to H2AZ is H2Av. H2Av is an essential protein in Drosophila and has been implicated in both activation and repression of transcription. H2Av is localized to centromeric heterochromatin in Drosophila and flies lacking H2Av have reduced levels of heterochromatin components at the centromeres. However, H2Av nucleosome distribution throughout the rest of the Drosophila genome correlates with genes that have an open and uniform chromatin architecture at promoter regions. Histone H2Av antibody (mAb) (Clone 10E9.D1) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation and Western blot, it has been shown to react with Drosophila samples.

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a peptide in the C-terminus of the Drosophila melanogaster Histone variant H2Av.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for H2AFV

Target: H2AFV (H2A Histone Family, Member V (H2AFV))

Alternative Name: Histone H2Av

Molecular Weight: 15 kDa

NCBI Accession: NP_524519