Histone H3.3 antibody (pSer31)

Catalog No. ABIN6971843

This Rabbit Polyclonal antibody specifically detects Histone H3.3 in WB, IF and DB. It exhibits reactivity toward Human.

Quick Overview for Histone H3.3 antibody (pSer31) (ABIN6971843)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)

Product Details anti-Histone H3.3 Antibody

Binding Specificity: pSer31

Characteristics: Histone H3.3 (H3F3) is a variant of histone H3 that contains a serine (S) to alanine (A) replacement at amino acid position 31. This variant Histone H3.3 has been found to replace conventional histone H3 in nucleosomes of active genes. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Histone variants differ in amino acid sequence from their core histone counterparts and in some cases have also been shown to have functional differences to the more typical histones. Histone variant H3.3 is the predominant form of histone H3 in non-dividing cells. Phosphorylation of serine 31 of histone variant H3.3 is specific to regions bordering centromeres in metaphase chromosomes. (Reference: Hake et al. (2005) PNAS 102: 6344-6349.) Histone H3.3S31ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Affinity Purified

Immunogen: This Histone H3.3 phospho Ser31 antibody was raised against a peptide containing phospho Ser31 of Histone H3.3.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: Histone H3.3

Molecular Weight: 17 kDa

NCBI Accession: NP_002908