Histone H4 antibody (2meArg3 (asymetric))

Catalog No. ABIN6972142

This anti-Histone H4 antibody is a Rabbit Polyclonal antibody detecting Histone H4 in WB, IF, ChIP and DB. Suitable for Human.

Quick Overview for Histone H4 antibody (2meArg3 (asymetric)) (ABIN6972142)

Target: Histone H4

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

Product Details anti-Histone H4 Antibody

Binding Specificity: 2meArg3 (asymetric)

Characteristics: Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Asymmetric dimethylation of histone H4 at arginine 3 (Arg3), catalyzed by the PRMT methyltransferases, is associated with transcriptional activation by nuclear hormone receptors. Arg3 methylation has been reported to facilitate the subsequent acetylation of histone H4 by the acetyltransferase p300. Histone H4 dimethyl Arg3 asymmetric methylation (H4R2me2a) is catalyzed by PRMT1 and PRMT6. Histone H4R3me2a (asymmetric) antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Dot blot, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Protein A Chromatography

Immunogen: This Histone H4 dimethyl Arg3, asymmetric antibody was raised against a synthetic peptide containing dimethyl-Arg3 (asymmetric) of human histone H4.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified rabbit IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for Histone H4

Target: Histone H4

Molecular Weight: 8 kDa

NCBI Accession: NP_778224