IgE antibody

Catalog No. ABIN6976324

This Mouse Monoclonal antibody specifically detects IgE in ELISA, WB, IF and IHC (fro). It exhibits reactivity toward Sheep.

Quick Overview for IgE antibody (ABIN6976324)

Target: IgE

Reactivity: Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This IgE antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1E7H11

Product Details anti-IgE Antibody

Specificity: Sheep, bovine, goat IgE.

Cross-Reactivity (Details): Does not cross-react with IgM, IgA, IgG1 or IgG2

Purification: Protein G affinity purified

Purity: > 95 %

Immunogen: Recombinant sheep IgE1-2 expressed in E. coli

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS

Storage: 4 °C,-20 °C

Storage Comment: Store at 4 °C, short term (1-2 weeks). For long-term storage, aliquot and keep at or below -20° C. Avoid repeated freeze-thaw cycles

Target Details for IgE

Target: IgE

Background: IgE is an immunoglobulin primarily produced from plasma cells. In normal serum it is present at very low concentrations. Western blot analysis against affinity purified ovine IgE using Mouse anti Sheep IgE clone 1E7H11 demonstrates a single major band of approximately 80 kDa or 200 kDa under reducing and non-reducing conditions, respectively