GAPDH antibody (AbBy Fluor® 555)

Catalog No. ABIN6978901

This Mouse Monoclonal antibody specifically detects GAPDH in WB and FACS. It exhibits reactivity toward Human, Mouse, Rat and Pig.

Quick Overview for GAPDH antibody (AbBy Fluor® 555) (ABIN6978901)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is conjugated to AbBy Fluor® 555

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 4F8

Product Details anti-GAPDH Antibody (AbBy Fluor® 555)

Cross-Reactivity: Human, Mouse, Pig, Rat

Purification: Purified by Protein G.

Immunogen: KLH conjugated synthetic peptide derived from human GAPDH

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background:

Synonyms: Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, GAPD, CDABP0047, OK/SW-cl.12

Background: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Gene ID: 2597

UniProt: P04406