DAPK3 antibody (pThr265) (AbBy Fluor® 750)

Catalog No. ABIN6982484

The Rabbit Polyclonal anti-DAPK3 antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect DAPK3 in samples from Mouse and Rat.

Quick Overview for DAPK3 antibody (pThr265) (AbBy Fluor® 750) (ABIN6982484)

Target: DAPK3 (Death-Associated Protein Kinase 3 (DAPK3))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAPK3 antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-DAPK3 Antibody (AbBy Fluor® 750)

Binding Specificity: pThr265

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthesised phosphopeptide derived from human ZIP Kinase around the phosphorylation site of Thr265

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DAPK3

Target: DAPK3 (Death-Associated Protein Kinase 3 (DAPK3))

Alternative Name: ZIP Kinase

Background:

Synonyms: Death-associated protein kinase 3, DAPK3, DAP kinase 3, Dlk, MYPT1 kinase, Zipper-interacting protein kinase, ZIP-kinase, ZIPK

Background: Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.

Gene ID: 1613

UniProt: O43293