Histone H2B antibody (Biotin)

Catalog No. ABIN6983098

This Mouse Monoclonal antibody specifically detects Histone H2B in IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Histone H2B antibody (Biotin) (ABIN6983098)

Target: Histone H2B

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H2B antibody is conjugated to Biotin

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 4H9

Product Details anti-Histone H2B Antibody (Biotin)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein G.

Immunogen: KLH conjugated synthetic peptide derived from human Histone H2B

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for Histone H2B

Target: Histone H2B

Background:

Synonyms: H2B.1 A, H2B/a, H2B/g, H2B/h, H2B/k, H2B/l, H2B/l.

Background: In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA, making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. Whilst the core histones are highly conserved across a wide range of organisms, the linker histones are less conserved.

Gene ID: 3018

UniProt: P33778