Phosphotyrosine antibody (phosphorylated) (Cy5)

Catalog No. ABIN6984854

The Mouse Monoclonal anti-Phosphotyrosine antibody is suitable to detect Phosphotyrosine in samples from Human, Mouse and Rat. It has been validated for IF (cc).

Quick Overview for Phosphotyrosine antibody (phosphorylated) (Cy5) (ABIN6984854)

Target: Phosphotyrosine

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is conjugated to Cy5

Application: Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 4D3

Product Details anti-Phosphotyrosine Antibody (Cy5)

Binding Specificity: phosphorylated

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein G.

Immunogen: KLH conjugated Phosphotyrosine

Isotype: IgG

Application Details

Application Notes: IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Target Type: Amino Acid

Background:

Synonyms: Phospho-tyrosine, Phospho tyrosine, Phospho-tyr, Phospho tyr, p-Tyr, p-tyrosine,

Background: The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.