E2F3 antibody (AA 91-190) (Cy5.5)

Catalog No. ABIN6985528

This Rabbit Polyclonal antibody specifically detects E2F3 in WB, IF (cc) and IF (p). It exhibits reactivity toward Mouse and Rat.

Quick Overview for E2F3 antibody (AA 91-190) (Cy5.5) (ABIN6985528)

Target: E2F3

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F3 antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-E2F3 Antibody (Cy5.5)

Binding Specificity: AA 91-190

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human E2F3

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for E2F3

Target: E2F3

Background:

Synonyms: DKFZp686C18211, E2F 3, E2F transcription factor 3, KIAA0075, MGC104598, Transcription factor E2F 3, E2F3_HUMAN, Transcription factor E2F3, E2F-3.

Background: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. [provided by RefSeq].

Gene ID: 1871

UniProt: O00716