EDC4 antibody (AA 1-100) (Cy7)

Catalog No. ABIN6985826

The Rabbit Polyclonal anti-EDC4 antibody has been validated for IF (cc) and IF (p). It is suitable to detect EDC4 in samples from Human, Mouse and Rat.

Quick Overview for EDC4 antibody (AA 1-100) (Cy7) (ABIN6985826)

Target: EDC4 (Enhancer of mRNA Decapping 4 (EDC4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EDC4 antibody is conjugated to Cy7

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-EDC4 Antibody (Cy7)

Binding Specificity: AA 1-100

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EDC4

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EDC4

Target: EDC4 (Enhancer of mRNA Decapping 4 (EDC4))

Alternative Name: EDC4

Background:

Synonyms: autoantigen, Autoantigen Ge 1, Autoantigen Ge-1, Autoantigen Ge1, Autoantigen RCD 8, Autoantigen RCD-8, Autoantigen RCD8, edc4, EDC4_HUMAN, Enhancer of mRNA decapping 4, Enhancer of mRNA-decapping protein 4, Ge 1, Hedls, Human enhancer of decapping large subunit, RCD 8.

Background: The major eukaryotic mRNA decay pathway occurs through deadenylation, decapping, and 5' to 3' degradation of the mRNA. Decapping is a critical control point in this decay pathway. Edc4 (enhancer of mRNA decapping 4), also known as human enhancer of decapping large subunit (HEDLS), RCD-8 or Ge-1, is a 1,401 amino acid protein belonging to the WD repeat EDC4 family that is involved in mRNA decapping during mRNA degradation. As part of the mRNA degradation process, Edc4 becomes part of a complex that also contains hDcp1a, hDcp2a, RCK and Edc3. Localizing to P-body and cytoplasm, Edc4 contains a nuclear localization sequence (NLS) which enables it to selectively enter the nucleus as well. Edc4 becomes phosphorylated upon DNA damage and exists as two alternatively spliced isoforms that are encoded by a gene that maps to human chromosome 16q22.1.

Gene ID: 23644

UniProt: Q6P2E9