EIF4A2 antibody (AA 141-240) (Cy7)

Catalog No. ABIN6985937

The Rabbit Polyclonal anti-EIF4A2 antibody has been validated for IF (cc) and IF (p). It is suitable to detect EIF4A2 in samples from Rat.

Quick Overview for EIF4A2 antibody (AA 141-240) (Cy7) (ABIN6985937)

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4A2 antibody is conjugated to Cy7

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-EIF4A2 Antibody (Cy7)

Binding Specificity: AA 141-240

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human eIF4A2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EIF4A2

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Alternative Name: eIF4A2

Background:

Synonyms: ATP dependent RNA helicase eIF4A 2, BM-010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F, Eukaryotic initiation factor 4A II, eukaryotic translation initiation factor 4A isoform 2, Eukaryotic translation initiation factor 4A2, IF4A2_HUMAN.

Background: Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a 5-cap motif and recruits the 43S ribosomal preinitiation complex to the eligible transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5 cap, eIF4A(I,II) are bidirectional RNA helicases, and eIF4G(I,II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3 and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7 % homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2.

Gene ID: 1974

UniProt: Q14240