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Histone 3 antibody (H3K27ac) (Cy7)

This Rabbit Polyclonal antibody specifically detects Histone 3 in WB, IF (cc) and IF (p). It exhibits reactivity toward Human and Rat.
Catalog No. ABIN6986539

Quick Overview for Histone 3 antibody (H3K27ac) (Cy7) (ABIN6986539)

Target

See all Histone 3 (H3) Antibodies
Histone 3 (H3) (Histone H3 (H3))

Reactivity

  • 1650
  • 1118
  • 989
  • 39
  • 39
  • 37
  • 34
  • 32
  • 25
  • 23
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  • 19
  • 11
  • 6
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  • 3
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  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Rat

Host

  • 1440
  • 237
  • 8
  • 5
Rabbit

Clonality

  • 1100
  • 589
  • 1
Polyclonal

Conjugate

  • 870
  • 102
  • 67
  • 67
  • 67
  • 67
  • 67
  • 67
  • 29
  • 27
  • 24
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  • 18
  • 18
  • 18
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  • 17
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This Histone 3 antibody is conjugated to Cy7

Application

  • 1302
  • 567
  • 486
  • 390
  • 333
  • 292
  • 203
  • 201
  • 189
  • 187
  • 176
  • 125
  • 113
  • 48
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  • 37
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  • 26
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  • 15
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  • 7
  • 7
  • 7
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
  • Binding Specificity

    • 71
    • 51
    • 50
    • 49
    • 46
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    • 45
    • 45
    • 44
    • 38
    • 38
    • 36
    • 33
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    • 29
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    • 28
    • 25
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    • 10
    H3K27ac

    Cross-Reactivity

    Human, Rat

    Purification

    Purified by Protein A.

    Immunogen

    KLH conjugated Synthesised acetylpeptide derived from human Histone H3 around the acetylation site of K27

    Isotype

    IgG
  • Application Notes

    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Buffer

    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    Histone 3 (H3) (Histone H3 (H3))

    Alternative Name

    Histone H3

    Background

    Synonyms: acetyl H3, Histone H3(acetyl k27), H3K27, H 3, H3 3 Like Sequence MH921, H3 3A, H3, H3 Histone, H3 Histone Family Member E Pseudogene, H3.4, H3/A, H3/g, H31_HUMAN, H3F3, H3FA, H3FT, H3t, HIST1H3J, HIST3H3, Histone cluster 1, H3a, Histone H3 3 Pseudogene, Histone H3.1, Histone H3.3, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l,

    Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

    Gene ID

    8290

    UniProt

    P68431
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