EPAS1 antibody (AA 1-130) (PE)

Catalog No. ABIN6988372

Product Details anti-EPAS1 Antibody (PE)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Binding Specificity: AA 1-130

Reactivity: Human, Rat, Mouse, Monkey, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPAS1 antibody is conjugated to PE

Application: Western Blotting (WB), Flow Cytometry (FACS)

Cross-Reactivity: Human, Monkey, Mouse, Rat, Sheep

Predicted Reactivity: Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse HIF2 Alpha

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: HIF2 alpha (EPAS1 Products)

Background:

Synonyms: HLF, HRF, MOP2, HIF2A, bHLHe73, HIF-2alpha, Endothelial PAS domain-containing protein 1, EPAS-1, HIF-1-alpha-like factor, mHLF, HIF-related factor, Hypoxia-inducible factor 2-alpha, HIF-2-alpha, HIF2-alpha, Epas1

Background: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP3. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity).

Gene ID: 13819

UniProt: P97481

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect