mCherry antibody (AA 1-236)

Catalog No. ABIN6989392

This Rabbit Polyclonal antibody specifically detects mCherry in WB. It exhibits reactivity toward Tag.

Quick Overview for mCherry antibody (AA 1-236) (ABIN6989392)

Target: mCherry (mCherry Fluorescent Protein)

Reactivity: Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This mCherry antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-mCherry Antibody

Binding Specificity: AA 1-236

Cross-Reactivity: Tag

Cross-Reactivity (Details): mCherry-Tag

Purification: Purified by Protein A.

Immunogen: Recombinant mCherry protein

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for mCherry

Target: mCherry (mCherry Fluorescent Protein)

Alternative Name: mCherry-Tag

Background:

Synonyms: mCherry Tag, monomeric cherry red fluorescent protein.

Background: mCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively. It is resistant to photobleaching and is stable. mCherry is sometimes preferred to other fluorophores due to its colour, as well as its photostability compared to other monomeric fluorophores.