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mCherry antibody

Name: mCherry antibody
Brand: antibodies-online
SKU: ABIN7505996
Availability: InStock

The Rabbit Polyclonal anti-mCherry antibody (ABIN7505996) specifically detects mCherry in WB, ICC and FACS. The antibody is reactive with Anaplasma marginale samples.

Western blotting analysis of mCherry fluorescent protein using rabbit polyclonal PAb (918) on lysates of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs (HEK293T/17 cells transfected with YFP/GPI, negative control) under reducing and non-reducing conditions. (mCherry antibody)

Flow cytometry surface staining pattern of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained using anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC). (mCherry antibody)

FACS

Separation of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mCherry/YFP cell suspension. (mCherry antibody)

FACS

3 images

Catalog No. ABIN7505996

$483.86

Plus shipping costs $50.00

100 μg

Shipping to: United States

Delivery in 5 to 9 Business Days

Quick Overview for mCherry antibody (ABIN7505996)

Target

mCherry (mCherry Fluorescent Protein)

Reactivity

Anaplasma marginale

Host

Rabbit

Clonality

Polyclonal

Conjugate

This mCherry antibody is un-conjugated

Application

Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose

Anti-mCherry purified

Specificity

The rabbit polyclonal antibody PAb (918) recognizes a red fluorescent protein tag mCherry.

Purification

Purified by ligand affinity chromatography.

Immunogen

mCherry protein from Anaplasma marginale
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Reactivity: Discosoma WB, IF, ICC Host: Rabbit Polyclonal unconjugated

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Reactivity: Discosoma WB, IF, ICC Host: Chicken Polyclonal unconjugated

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Reactivity: Discosoma WB, IP Host: Mouse Monoclonal unconjugated

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Reactivity: Discosoma WB, IF, ICC, IHC Host: Mouse Monoclonal 1C51 unconjugated

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Reactivity: Discosoma WB, IF, ELISA Host: Mouse Monoclonal 1A186 unconjugated

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Reactivity: Discosoma WB Host: Mouse Monoclonal unconjugated

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Reactivity: Various Species WB Host: Mouse Monoclonal unconjugated

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Reactivity: Discosoma, Tomato WB, IF, IHC (fro), IHC (p), EM Host: Goat Polyclonal unconjugated

Application Notes

Flow cytometry: Recommended dilution: 1-4 μg/mL, extracellular staining or intracellular staining - depending on expression.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store at 2-8°C. Do not freeze.
Target

mCherry (mCherry Fluorescent Protein)

Alternative Name

mCherry

Background

The mCherry is a red fluorescent protein with excitation maximum 587 nm and emission maximum 610 nm. It has around 28 kDa, and it is being used as a fluorescent tag in expression systems.

UniProt

X5DSL3