SMAD2 / SMAD3 (pThr8) antibody

Catalog No. ABIN6989419

This Rabbit Polyclonal antibody specifically detects in WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SMAD2 / SMAD3 (pThr8) antibody (ABIN6989419)

Target: SMAD2 / SMAD3

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Product Details

Binding Specificity: pThr8

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthesised phosphopeptide derived from human Smad2/Smad3 around the phosphorylation site of Thr8

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
IF()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: SMAD2 / SMAD3

Alternative Name: Smad2/Smad3

Background:

Synonyms: Smad2 + Smad3 (phospho T8), p-Smad2 + Smad3 (phospho T8), P-Smad2 /3 (phospho T8), hMAD 2, hMAD 3, hSMAD2, hSMAD3, Mad related protein 2, MADH2, MADH3, MADR2, Mothers against DPP homolog 2, Mothers against DPP homolog 3, Sma and Mad related protein 2, SMA and MAD related protein 3, SMAD 2, SMAD 3, SMAD family member 2, SMAD family member 3.

Background: SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.

Gene ID: 4087

UniProt: P84022